Figure 3.

Analysis of thylakoid protein complexes from bfa3 and wild-type plants. A, Immunodetection of thylakoid proteins from bfa3 and WT plants. Thylakoid proteins were loaded on an equal chlorophyll basis. B, BN gel analysis of thylakoid membrane protein complexes. The BN gel (left) was further stained with Coomassie Brilliant Blue (CBB; right). NDH-PSI, NDH-PSI supercomplex. C, 2D BN/SDS-PAGE separation of thylakoid protein complexes. Complexes separated by BN-PAGE (B) were resolved into their constituent subunits by SDS-urea-PAGE. Proteins were stained with CBB; the identities of relevant proteins are indicated. The position of intact chloroplast ATP synthases (CF₁-CF₀) and the CF₁ subcomplex (CF₁) in the first dimension are indicated with arrows. The complex between the CF₁-CF₀ ATP synthase and CF₁ subcomplex (marked with an asterisk) is a putative complex containing the β-subunit.