Figure 7.

Characterization of BFA3. A, Phylogenetic tree of BFA3 proteins. The sequences were retrieved from GenBank, and evolutionary analyses were conducted in MEGA5 using the neighbor-joining method. B, Transient expression of BFA3-GFP in Arabidopsis protoplasts. Arabidopsis protoplasts were transformed with various vectors and examined by confocal microscopy. Bars = 5 μm. C, Immunodetection of BFA3. Thylakoid and stromal proteins isolated from bfa3 and WT plants were separated by SDS-urea-PAGE and immunodetected with polyclonal antisera against BFA3. D1 and RbcL were used as fractionation and loading controls.