Figure 6.

The UV-B photoreceptor UVR8 is required for SAR. A and B, Standard SAR assays of 4-week-old Col-0, uvr8-6, and 35S:UVR8 plants. Leaves were mock inoculated (10 mm MgCl₂) or induced for SAR by pressure infiltration with 10⁶ cfu mL⁻¹ Pst DC3000/avrRpt2. Two days later, distant leaves were challenged with 10⁶ cfu mL⁻¹ Pst DC3000, and Pst levels in these leaves were quantified 3 dpi. This experiment was performed six times, with similar results observed three times each. C, Local resistance assays of Col-0, uvr8-6, and 35S:UVR8 to virulent (VIR; Pst DC3000) and avirulent (AVIR; Pst DC3000/avrRpt2) strains of Pst. Leaves of 4-week-old plants were inoculated with 10⁶ cfu mL⁻¹ of either strain, and in planta bacterial density was calculated at 0 and 3 dpi. This experiment was performed three times with similar results. All values represent means ± sd of three sample replicates. Different letters indicate statistically significant differences (ANOVA, Tukey’s HSD, P < 0.05).