Table II. Summary of CaDGAT1 variant libraries, and approximate number of yeast strains screened by Nile Red staining, with or without prior enrichment of low-density strains.
| Parameter | Library | |||||
|---|---|---|---|---|---|---|
| A |
B |
C |
D |
E |
F |
|
| No. of planned amino acid substitutions | 36 | 17 | 19 | 23 | 11 | 12 |
| Theoretical size of library (planned substitutions only) | 6.9 × 1010 | 1.3 × 105 | 5.2 × 105 | 8.4 × 106 | 2,048 | 4,096 |
| No. of variants amplified in E. coli | 4,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 |
| No. of yeast strains screened by Nile Red staining, without prior low-density enrichment | 200 | 200 | 200 | 600 | 1,300 | 0 |
| No. of yeast strains enriched for low density | 5,000 | 6,000 | 10,000 | 7,000 | 0 | 8,000 |
| No. of yeast strains screened by Nile Red staining, following low-density enrichment | 700 | 400 | 400 | 600 | 0 | 900 |