Table IV. Kinetic parameters of CaDGAT1 variants that gave high oil content in yeast.

Means ± se were determined by the SigmaPlot 12.5 enzyme kinetics module. ND, Not determined.

DGAT Variant	S0.5	Vmax	DGAT Protein Abundance in Microsomes (Relative to CaDGAT1)	Vmax, Adjusted for Protein Abundance		Hill Coefficient
	µm 18:1-CoA	pmol min−1 mg−1 microsomal protein		pmol min−1 mg−1 protein	% of CaDGAT1
CaDGAT1	0.94 ± 0.12	3,798 ± 284	1	3,798	100	1.8 ± 0.38
CaDGAT1-C11	0.20 ± 0.036	2,517 ± 301	0.2	12,585	331	2.6 ± 1.1
CaDGAT1-C10	0.39 ± 0.025	2,467 ± 70	0.83	2,972	78	2.5 ± 0.40
CaDGAT1-C13	0.52 ± 0.076	1,851 ± 124	0.29	6,383	168	2.3 ± 0.71
CaDGAT1-C9	0.53 ± 0.079	2,210 ± 148	0.18	12,278	323	2.1 ± 0.62
CaDGAT1-E3	0.57 ± 0.088	2,295 ± 168	0.2	11,475	302	2.5 ± 0.81
CaDGAT1-D16	0.59 ± 0.075	1,989 ± 117	0.95	2,094	55	1.8 ± 0.45
CaDGAT1-D2	0.69 ± 0.076	3,003 ± 163	1.25	2,402	63	2.1 ± 0.48
CaDGAT1-D19	0.75 ± 0.11	3,133 ± 246	0.6	5,222	137	2.5 ± 0.86
CaDGAT1-A2	1.11 ± 0.20	7,584 ± 737	0.92	8,243	217	1.6 ± 0.46
ZmDGAT1-2	2.8 ± 0.19	1,138 ± 61	ND	ND	ND	2.7 ± 0.44