Table V. Composition of T1 seeds expressing GmDGAT1b-MOD.
A total of four transgenic events expressing GmDGAT1b-MOD were generated using particle bombardment and recombination-mediated cassette exchange. For every event, two T0 plants were generated and grown to maturity in controlled environments. Thirty-six seeds from every T0 source were subjected to nondestructive compositional analysis using single-seed near-infrared reflectance spectroscopy (SS-NIR). In this manner, 72 seeds from each of the four events were analyzed. Seeds were classified as null or transgenic based on oleic acid and total oil contents. Mean comparisons that were not significant at the 5% level (probability t test, homoscedastic Student’s t test P ≤ 0.05; Microsoft Excel) are indicated by ns. TG, Transgenic segregant; Δ % pts, change in percentage points.
| Event | Percentage Oleic Acid (18:1) |
Percentage Oil |
Percentage Protein |
χ2 |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Null | TG | Δ % pts | Null | TG | Δ % pts | Null | TG | Δ % pts | ||
| Soil 111 SOY 3615.1.1 | 22.7 | 32.7 | 10.0 | 18.3 | 23.6 | 5.3 | 40.6 | 39.3 | −1.3 (ns) | 1.7 |
| Soil 111 SOY 3615.2.2 | 18.3 | 28.4 | 10.1 | 19.6 | 24.4 | 4.8 | 33.6 | 34.6 | 1.0 (ns) | 0.3 |
| Soil 111 SOY 3615.4.1 | 18.2 | 29.2 | 10.9 | 17.8 | 22.9 | 5.1 | 36.8 | 37.5 | 0.7 (ns) | 0.6 |
| Soil 111 SOY 3615.9.1 | 20.2 | 30.6 | 10.3 | 19.2 | 24.3 | 5.1 | 36.8 | 37.6 | 0.8 | 1.2 |
| All event average | 19.9 | 30.2 | 10.3 | 18.7 | 23.8 | 5.1 | 37.0 | 37.3 | 0.3 | |