Figure 5.

Increased callose accumulation and decreased plasmodesmata conductivity of atgpi8-1. A and B, The accumulation of callose in 17-d-old cotyledons of atgpi8-1 (B) is increased compared to wild type (A) as determined by aniline blue staining. C and D, Analysis of GFP movement in the epidermis of 7-d-old seedlings suggests decreased plasmodesmata conductivity in atgpi8-1. C, Representative images of the abaxial side of the epidermis expressing cobombarded ER-localized RFP (left), GFP (center), and both merged (right). D, Distribution analysis of the number of cells in clusters expressing GFP from a single transformation event based on RFP expression; n = 40, P < 0.0001.