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. 2016 Apr 18;171(2):1418–1426. doi: 10.1104/pp.16.00372

Figure 1.

Figure 1.

Reduction of endogenous CK confers As(V) tolerance. A, qRT-PCR analysis of PHT1;1 expression in wild-type plants (Col-0) and in a 35S:CKX1-overexpressing line grown in +Pi medium for 7 d, transferred to −Pi medium for 3 d, then transferred to −Pi liquid medium for the indicated time, alone (−Pi) or with 30 μm As(V). Values show mean ± sd (n = 3). B, As(V) tolerance phenotypes of ipt 3 5 7, wild-type (Col-0), cyp735a1 a2, 35S:CKX1, and ahk2 ahk3 plants grown on 12.5 μm Pi supplemented with 25 μm As(V) for 10 d. C, Root length of indicated mutant, transgenic, and wild-type plants grown alone (left) or with As(V) (center) were measured in plants growth as described in B. Right, root growth inhibition (fold change) by As(V) compared to growth of corresponding plant types in the absence of As(V). Values show mean ± sd (n = 5). Means are significantly different (Student’s t test, *P < 0.01).