Figure 2.

As(V) depletes endogenous CKs. A, Wild-type plants (Col-0) were grown in +Pi medium for 7 d, transferred to -Pi medium for 3 d, and then to -Pi liquid medium alone or with 30 μm As(V) for 6 h. CK levels were analyzed as pmol/g fresh weight (FW) of whole plants. Values show mean ± sd (n = 3). Means are significantly different (ANOVA, *P < 0.05). B and C, As(V) inhibits expression of CK metabolism genes. qRT-PCR analysis of IPT (B) and CKX (C) gene expression. Treatment was as in A. Values show mean ± sd (n = 3), t test *P < 0.05.