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Phytochelatins, GSH, and As(III) accumulation increased in response to CK depletion in Arabidopsis and tobacco. GSH, PCs PC2, PC3, PC4 A, and As(III) (B) levels were determined in Col-0 and 35S:CKX1 Arabidopsis seedlings. Quantification of thiols (C) and As(III) (D) in wild-type (SNN) and W6::CKX1 tobacco roots. Phytochelatins and As(III) were quantified as described in “Methods.” Values show mean ± sd (n = 3). *P < 0.05 (Student’s t test). FW, fresh weight; DW, dry weight.
