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. 2016 Apr 20;171(2):1320–1332. doi: 10.1104/pp.16.00458

Figure 4.

Figure 4.

Analysis of the NDH-1 complex in different NDH-1 mutant backgrounds. A, Immunoblot analysis of Ndh subunits in thylakoid membranes of wild-type, ndhM, ndhN, ndhO, ndhH, and ndhJ deletion mutants. Each lane was loaded with thylakoid membrane proteins corresponding to 2 μg chlorophyll a, and the series of dilution is indicated. In the lower panel, a replicated gel stained with Coomassie Brilliant Blue (CBB) was used as a loading control. B, BN-PAGE analysis of thylakoid protein complexes isolated from wild-type, ndhN, ndhO, ndhH, and ndhJ deletion mutants. Each lane was loaded with thylakoid membrane proteins corresponding to 3 μg chlorophyll a.