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Analysis of the cytoplasmic intermediate complexes isolated from the wild type and various NDH-1 mutant strains. A to E, Cytoplasmic protein complexes isolated from the wild type and the various mutants were separated by CN-PAGE and further subjected to 2D/SDS-PAGE. The proteins were immunodetected with antibodies against Ndh subunits (NdhH, NdhI, NdhJ, NdhK, NdhM, and NdhN). The positions of molecular mass markers are indicated.
