Table I. Oxygen exchange rates of wild-type and mutant cells.
Conditions | Parameters | Components | Wild Type | ∆cyd | ∆cox | ∆cox/cyd |
---|---|---|---|---|---|---|
Control | Oxygen uptake | Dark | 8.3 ± 1.2 | 5.1 ± 0.8* | 3.5 ± 0.5* | 0.3 ± 0.2* |
Light induced | 26.3 ± 6.9 | 35.3 ± 8.3* | 38.4 ± 5.5* | 24.7 ± 3.0 | ||
Total light | 34.6 ± 6.7 | 40.5 ± 7.5 | 41.5 ± 5.1* | 24.9 ± 3.0 | ||
Oxygen production | Gross | 193.3 ± 20.1 | 190.7 ± 26.4 | 190.25 ± 18.1 | 184.9 ± 21.5 | |
Net | 158.6 ± 19.3 | 150.3 ± 19.2 | 148.7 ± 15.1 | 159.6 ± 22.5 | ||
DBMIB | Oxygen uptake | Dark | 10.1 ± 1.5 | 13.1 ± 1.3 | 12.8 ± 3.1 | 9.3 ± 1.3 |
Light induced | 11.6 ± 2.3 | N/A | 7.9 ± 0.6 * | N/A | ||
Total light | 21.5 ± 2.8 | 7.0 ± 0.8* | 20.7 ± 2.5 | 7.7 ± 1.9* | ||
Oxygen production | Gross | 24.2 ± 3.1 | 5.5 ± 1.3* | 19.8 ± 3.8* | 2.75 ± 0.4* | |
Net | 2.9 ± 0.2 | N/A | N/A | N/A | ||
DBMIB + HQNO | Oxygen uptake | Dark | 12.9 ± 0.3 | 11.3 ± 3.5 | 10.9 ± 3.9 | 10.2 ± 0.2* |
Light induced | N/A | N/A | N/A | N/A | ||
Total light | 12.3 ± 0.6 | 7.0 ± 1.1 | 10.8 ± 3.4 | 9.8 ± 0.5 |