Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 11;9(3):149–157. doi: 10.1111/cts.12399

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors. Clinical and Translational Science published by Wiley Periodicals, Inc. on behalf of American Society for Clinical Pharmacology and Therapeutics

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Effect of reduced cellular nicotinamide phosphoribosyltransferase (NAMPT) expression on the pharmacological activity of methotrexate (MTX). A549 cells were treated with either siRNA targeting NAMPT mRNA or a scrambled control siRNA and evaluated after 96 hours for differences in (a) NAMPT expression by Western blot analysis, (b) intracellular nicotinamide adenine dinucleotide (NAD) levels, (c) cell viability measured as resorufin relative fluorescence units (RFUs), and (d) sensitivity to the growth inhibitory effects of MTX. Results from a single experimental evaluation including three independent replicates are presented here along with the resulting mean ± SD. The Western blot is representative of three independent experimental evaluations and the MTX growth inhibitory response data is representative of four independent experimental evaluations.