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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol Methods. 2016 Apr 16;434:53–60. doi: 10.1016/j.jim.2016.04.007

Figure 2. Correlations between different methods for quantification of HIV-1 replication.

Figure 2

Frequency of HIV-1 p24+ CD4+ T cells, p24 Gag concentration and HIV-1 RNA copy numbers in the supernatant of all individuals (n=13) and conditions were plotted against each other. Percentage of p24+ CD4+ T cells was assessed by flow cytometry, p24 Gag production was determined by ELISA and viral RNA was quantified by RT-qPCR respectively. Panel A displays absolute values of HIV-1 replication (52 data points); panel B shows log inhibition of HIV-1 replication as compared to CD4+ T cells alone (39 data points). Non-parametric spearman’s rank correlation was used to determine association between two methods.