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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Life Sci. 2015 Dec 10;159:140–143. doi: 10.1016/j.lfs.2015.12.022

Fig. 2. The basic strategy for isolation and imaging of the renal vessels.

Fig. 2

(A) Dissecting of renal resistance arteries. (B) Imaging renal vessels with two photon microscopy allows detecting three distinct tissue layers at different depths within the vessel wall: 1, fibroblasts rich in collagen; 2, smooth muscle cells (SMC) and 3, endothelial cells/elastic fibers. (C) Example of renal vessel imaging loaded with Fluo-4 AM Ca2+ indicator: Z-stack of the vessel, TL – vessel in transmitted light, 1, 2, and 3 corresponding layers are described in B.