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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: ACS Chem Biol. 2016 Feb 10;11(4):1137–1147. doi: 10.1021/acschembio.5b00913

Figure 7.

Figure 7

Proposed steps during substrate binding and conformational changes in the middle domain during catalysis. Substrate binding induces a conformational change in the middle domain of BexE, which causes FAD to move from the “in” to the “out” position. When FAD is in the “out” position, NAPDH reduction occurs and FAD returns to the “in” position. The reduced FAD reacts with molecular oxygen to form a reactive C4a peroxy-flavin intermediate. The reactive C4a peroxy-flavin intermediate delivers oxygen to the substrate and the product is released.