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. 2016 Jun 10;16:133. doi: 10.1186/s12870-016-0826-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Most contrasting HPLC_DAD carotenoids profiles. Carotenoid profiles for 3 of the 4 most contrasting HPLC_DAD carotenoid profiles from a total of 23 landraces studied. Carotenoid profiles were obtained by HPLC_DAD using a C18 Waters Spherisorb ODS_2 (4.6 × 150 mm, 5 mm) column and mobile phase of Methanol: Ethyl Acetate: Acetonitrile (1:1:8) with a flow rate of 1 mL per minute during 60 min in an isocratic run. Carotenoid types were identified using reference profiles (Additional file 3: Figure S2) and standard profiles (Additional file 4: Figure S3)