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. 2016 Jun 10;16:133. doi: 10.1186/s12870-016-0826-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Proposed diagram for carotenoid biosynthesis pathway in cassava storage root based on qRT_PCR gene expression analysis. Carotenoid biosynthesis pathway was designed based on carotenoid intermediates identified by isocratic run using a C18 column separation with HPLC_DAD identification system, abundance of transcripts coding for proteins/enzymes, and their code identification in the cassava genome. Analytical and diagram assembly were performed as described in Methods