Table 4.
Correlation parameters revealed among different classes of carotenoid types and abundance of transcripts coding for six major enzymes based on qRT_PCR
| Total acyclic carotenoid correlation | |||
|---|---|---|---|
| Enzyme | cDNA code in microarray data base | Cassava genome code | p value |
| ZDS | CV03051B1A05.f1 | cassava4.1_004265m | 0.109 |
| Total β-carotene correlation | |||
| LCYe | CV03088A2B02.f1 | cassava4.1_005406m | 0.067 |
| NCED3 | CV03105A1F08.f1 | cassava4.1_029535m | 0.046 |
| PDS | CV03049A2F07.f1 | cassava4.1_004359m | 0.037 |
| PDS | CV03110A2H07.f1 | 0.139 | |
| ZEP | CV03090B2B05.f1 | 0.003 | |
| ZEP | CV03104A2A10.f1 | 0.009 | |
| Total Xanthophyll correlation | |||
| LCYe | CV03127A2F10.f1 | 0.007 | |
Carotenoid intermediates were pooled based on classes of carotenoid end products including cyclic (β-carotene + β-xanthophylls), and acyclic (phytoene + phytofluene + ζ-carotene + neurosporene + β-zeacarotene + lycopene) carotenoids. Pearson product moment correlation coefficient (r) was obtained for linear association of total cyclic, total acyclic, total β − carotene and total xanthophylls against abundance of transcripts coding for enzymes related to carotenoids synthesis. The values of SE of r accounts for the standard error of the estimates by the linear regression and represents the level of accuracy of predictions. If probability that r = 0, P(r = 0) is < =0.05, r is significantly different from 0 and some degree of correlation is shown. The value n refers to number of replication