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. 2016 Jun 10;16:132. doi: 10.1186/s12870-016-0813-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Quantitative real-time PCR analysis of CtF3H relative expression of flower tissue in ZHH0119 line (a) and XHH007 line (b) at different time points post-treatment with MeJA. Each plant was individually assayed in triplicate (n = 4). Relative expression of CtF3H gene is normalized to Ct60s as control and compared with the relative expression without MeJA treatment at different time points using 2-^△△Ct method. Asterisks indicate a significant difference from 0 h (*:p < 0.05, **:p < 0.01)