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. 2016 Jun 10;15:46. doi: 10.1186/s12943-016-0531-5

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© Chiu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Combined treatment with YCW1 and IR enhances the anti-tumor effects in an orthotopic breast cancer model. a 4 T1-luc cells were injected into the mammary fat pads of Balb/c mice, observed for luciferase signals and photographed using an IVIS 200. b Measurement of body weight in Balb/c mice taken once per week. c Quantification of the luciferase signals. *, p < 0.05, versus control. d IHC staining of orthotopic tumor tissues from the mice. IHC was used to determine the expression levels of LC3 and BNIP3 (×100 objective magnification). The percentage of LC3 and BNIP3-positive cells was determined using HistoQuest software (TissueGnostics)