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. 2016 Jun 10;35:91. doi: 10.1186/s13046-016-0369-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Macroscopic and microscopic examination of organs with metastatic lesions and orthotopic pancreatic tumours. 24 mice were randomly divided into three groups (PANC-EV, PANC-MUC4/Y, PANC-MUC4/Y-AMOP^Δ). The different groups PANC-1 derived cells (2 × 10⁶ cells/100 μl) were orthotopic implanted into pancreas. Forties five dayed later, mice were euthanised by CO₂ asphyxiation and autopsied (a) The macroscopic examination of organs with metastatic lesions (includes liver, spleen, lung, peritoneum, and mesentery), malignant ascites and orthotopic pancreatic tumours. The arrows pointed the metastatic nodes. b H&E staining was used to the histological change. Normal (N) and tumour lesions (T) in different organs are separated as indicated