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. Author manuscript; available in PMC: 2017 Mar 18.
Published in final edited form as: Org Lett. 2016 Mar 9;18(6):1446–1449. doi: 10.1021/acs.orglett.6b00380

Figure 2.

Figure 2

In vitro characterization of CazI and CazL. (a) HPLC analysis of CazI using 1 as a substrate. HPLC traces were monitored at 254 nm. (i) Negative control with 1 lacking CazI. (ii) Reaction containing CazI and 1 incubated for 16 h at room temperature. (iii) Authentic reference of 2. (b) HPLC analysis of CazL using 2 and 1 as substrates. HPLC traces were monitored at 280 nm. (i) Negative control with 2 lacking CazL. (ii) Reaction containing CazL and 2 after 1 h at 30 °C. (iii) Negative control with 1 lacking CazL. (iv) Reaction containing CazL and 1 incubated for 1 h at 30 °C.