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. Author manuscript; available in PMC: 2017 Mar 18.
Published in final edited form as: Org Lett. 2016 Mar 9;18(6):1446–1449. doi: 10.1021/acs.orglett.6b00380

Figure 3.

Figure 3

HPLC analyses of in vitro assays on CazP and CazO. All HPLC traces were monitored at 280 nm. (a) In vitro characterization of CazP using 4 as a substrate. (i) Negative control with 4 lacking CazP. (ii) Reaction containing CazP and 4 incubated for 15 min at 30 °C. Authentic references of (iii) 4, (iv) 6 and (v) 8 are also shown. (b) Proposed reaction mechanism of lactonization from 4 in 6 via 8 by CazP. (c) In vitro characterization of CazP using 10 as a substrate. (i) Negative control with 10 lacking CazP. (ii) Reaction containing CazP and 10 incubated for 3 h at 30 °C. (d) In vitro characterization of CazO using 8 as a substrate. (i) Negative control with 8 lacking CazO. Most of 8 has converted into 6 spontaneously in the reaction mixture. (ii) Reaction containing CazO and 8 incubated for 1 h at 30 °C.