Table 2.
Selected MS parameters for the analysis of selected endocannabinoids and congeners, as reported by Richardson et al. (referenced material includes table heading) [38].
Analyte | Retention time (min) |
Precursor ion (m/z value) |
Product ion (m/z value) |
Cone voltage (eV) |
Collision energy (eV) |
---|---|---|---|---|---|
Palmitoyl ethanolamide | 6.13 | 300.26 | 62.00 | 35 | 15 |
Heptadecanoyl ethanolamide | 7.15 | 314.47 | 62.00 | 60 | 20 |
Oleoyl ethanolamide | 6.51 | 326.39 | 62.00 | 60 | 20 |
Virodhamine | 1.79 | 348.33 | 62.00 | 35 | 11 |
Anandamide | 5.43 | 348.33 | 62.00 | 35 | 11 |
2-Linoleoyl glycerol | 6.32 | 355.52 | 263.18 | 55 | 15 |
Arachidonyl ethanolamide-d8 | 5.43 | 356.33 | 62.00 | 48 | 21 |
Arachidonyl glycine | 7.85 | 362.24 | 287.11 | 50 | 15 |
Noladin ether | 6.51 | 365.20 | 273.17 | 63 | 14 |
2-Arachidonyl glycerol | 6.26 | 379.24 | 287.02 | 55 | 15 |
2-Arachidonyl glycerol-d8 | 6.26 | 387.35 | 96.17 | 43 | 34 |
Selected precursor and product m/z values with appropriate retention times, cone and collision voltages used to identify and quantify analytes, using MRM with the LC-MS-MS method.