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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Brain Struct Funct. 2015 Dec 11;221(9):4319–4335. doi: 10.1007/s00429-015-1163-z

Fig. 5.

Fig. 5

Alcohol dependence and nondependent drinking does not alter the phenotypic distribution of 29-day-old BrdU cells in the hippocampus and medial prefrontal cortex. (a–e, Hippocampus) Representative confocal z-stack images of 29-day-old BrdU+ cells (a, Cy3, red) colabeled with NeuN (b, FITC, green) and GFAP (c, CY5, blue). Arrowheads in a–d point to the BrdU+ cell; scale bar is 10 μm in panel (a), applies a–d. e Orthogonal view of the BrdU cell indicated in (a), shows that the cell is BrdU+/NeuN+ cell with both antibodies demonstrating equal penetration in xz and yz planes. f Quantitative analysis of BrdU-labeled cells in the hippocampus expressing NeuN or GFAP or neither in control, dependent (DEP) and nondependent (NON-DEP) rats [BrdU+/NeuN-/GFAP- (BrdU+ Only, solid bars), BrdU+/GFAP+/NeuN– (textured bars) and BrdU+/NeuN+/GFAP– (hashed bars)]. (g–k, mPFC) Representative confocal z-stack images of 29-day-old BrdU+ cells (g, Cy3, red) colabeled with Olig2 (h, FITC, green) and GFAP (i, CY5, blue). Arrowheads in g–j point to BrdU+/Olig2+ cell; arrow in H points to a Olig2+ cell that is not BrdU+. Scale bar in G is 10 μm, applies g–j. k Orthogonal view of the BrdU cell indicated in (g), shows that the cell is BrdU+/Olig2+ cell with both antibodies demonstrating equal penetration in xz and yz planes. l Quantitative analysis of BrdU-labeled cells in the mPFC expressing Olig2 or GFAP or neither in control, dependent (DEP) and nondependent (NON-DEP) rats [BrdU+/Olig2-/GFAP- (BrdU+ Only, solid bars), BrdU+/GFAP+/Olig2- (textured bars) and BrdU+/Olig2+/GFAP– (hashed bars)]