Table 2.
Species and genus specific primers used for real-time PCR.
| Gene | Primers (sense/antisense 5′-3′) | Size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| Total bacteria | F: ACTCCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG |
175 | 60 |
| Lactobacillus spp. | F: CACCGCTACACATGGAG R: TGGAAGATTCCCTACTGCT |
341 | 58 |
| Escherichia coli | F: CATGCCGCGTGTATGAAGAA R: TTTGCTCATTGACGTTACCCG |
96 | 60 |
| Enterococcus faecalis | F: CCCTTATTGTTAGTTGCCATCATT R: ACAATGGGAAGTACAACGAGT |
144 | 61 |
| TNF-α | F: CACCACGCTCTTCTGCCTACTG R: TTGAGACGATGATCTGAGTCCTTGG |
115 | 63 |
| MCP-1 | F: GTCCTTGCCCAGCCAGATG R: CGATGGTCTTGAAGATCACTGCT |
148 | 60 |
| IL-1β | F: AAAGGGGACTTGAAGAGAG R: CTGCTTGAGAGGTGCTGATGT |
286 | 58 |
| IL-6 | F: AAGGTGATGCCACCTCAGAC R: TCTGCCAGTACCTCCTTGCT |
151 | 60 |
| INF-γ | F: GAGCCAAATTGTCTCCTTCTAC R: CGAAGTCATTCAGTTTCCCAG |
140 | 61 |
| ZO-1 | F: GGCGCACGGCGAAGGTAATT R: CTATCAAACTCAGGAGGCGGCACT |
405 | 60 |
| Occludin | F: GGAGTGATTCGGATTCTGTCTATGCT R: CGCCTGGGCTGTTGGGTTGA |
423 | 60 |
| β-actin | F: CCAGGTCATCACCATCGG R: CCGTGTTGGCGTAGAGGT |
158 | 60 |