Fig. 2.

OGD increases p38 MAPK-dependent increases in superoxide generation in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of the p38 MAPK inhibitor, SB203580 (50 μm, 2 h prior to OGD). Slices were harvested at 0, 4, 8 and 24 h after OGD and subjected to EPR using the spin-trap compound 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine.HCl to determine superoxide levels. Representative EPR waveforms are shown (A). Absolute levels of superoxide generation were then determined as nmol superoxide generated / min per mg protein (B). Values are presented as mean + SE from four independent experiments using 12 pooled slices per experiment. *P < 0.05 vs. 0 h, ^†P < 0.05 vs. previous time-point, ^‡P < 0.05 vs. without SB20358 at the same time-point.