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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: Bioorg Med Chem Lett. 2016 May 12;26(14):3359–3363. doi: 10.1016/j.bmcl.2016.05.034

Figure 1. Design of photo-activatable protein labeling via singlet oxygen.

Figure 1

The interacting protein in close proximity to the protein of interest (POI) is labeled. POI is fused to singlet oxygen generator (SOG) to generate the singlet oxygen upon irradiation. Then, the thiol-containing labeling molecule (Y=SH) forms a disulfide bond with a surface cysteine (X=SH) residue of interacting protein. The labeled protein can then be purified by affinity column and released by hydroxylamine followed by trypsin digestion and identified by mass spectrometry.