ErbB2, septin-9, septin-2, and septin-7 expression were determined by western blot analysis of total lysates of various cell lines and human gastric antrum, β-actin was used as a control. Cofilin was used as a control for septin-2 given the size similarity between septin-2 and β-actin. ErbB2 is very highly expressed in HGE-20 gastric cancer cells, to the extent that the band is not visible in other cell types with reasonable exposure of the HGE-20 cell band. Once HGE-20 cells were removed from the ErbB2 blot, expression was also seen in human gastric antrum, HB2 breast cancer cells, and AGS gastric cancer cells. Septin-9 similarly showed increased expression in gastric cancer cells compared to other cell types.