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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Stem Cell Res. 2016 Apr 13;16(3):712–724. doi: 10.1016/j.scr.2016.04.008

Figure 3. CEMA selected gene sets can induce at least partial reprogramming.

Figure 3

(A) Schematic illustration of experimental design and CEMA selected genes cassettes (B–E), Reprogrammed NHDF–iNPC (B) NHDF-iNPC cells, sorted for YFP+ and treated with dox exhibit distinct morphology changes. The images shown are from a representative experiment, selected from over four separate experiments. (C) Some NHDF-iNPCs acquired neuronal morphology and stained positive for MAP2. Bars represent 50 microns. (D) NHDF-iNPCs expressed various neuronal and NPC markers as measured by quantitative real time PCR relative to NHDF-GFP; GAPDH expression was used for normalization. Error bars represent standard error of the mean of four separate reprogramming experiments, one tissue-NPC sample was used for comparison (E) Venn diagram of genes of which expression level changed 2 fold in NHDF-iNPC, NHDF-iNPC in which Dox was withdrawn (wd) compared to NHDF-GFP.