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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cell Biochem Biophys. 2016 Jan 27;74(2):191–203. doi: 10.1007/s12013-016-0719-8

Fig. 2.

Fig. 2

Functional analyses of NHBE cells in inserts grown under different culture conditions. TEER values were measured during the cultures in different conditions; (A) NHBEs in the growth medium (culture conditions were labeled as AIC or LCC) (B) NHBEs in the differentiation medium (AIC or LCC). TEER values of the differentiated NHBEs under AIC and LCC began to show the statistically significant differences from day 6 (unpaired Student's t-test with the 5% significance level. *P<0.05, **P<0.01, ***P<0.001). (C, D) Lucifer Yellow (LY) permeability (Peff) measurements on Day 8 for NHBE in the (C) growth or (D) differentiation medium. Apical-to-basolateral (AP→BL) or basolateral-to-apical (BL→AP) permeability values of NHBE cells on polyester membranes after the LY transport are depicted according to medium compositions and culture conditions (ALC or LCC).