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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Stem Cell Res. 2016 Mar 29;16(3):640–650. doi: 10.1016/j.scr.2016.03.009

Figure 2. Adaptation of hPSCs to non-colony type culture.

Figure 2

(A) Colony-type culture of hiPSC showing area of loss of pluripotency marker OCT4 expression.

(B) Colony-type culture of H9ESC and hiPSCs were adapted to a non-colony type culture, by serial passages at a single-cell level, using gentle dissociation buffer and high concentration of Rock Inhibitor (Y-27632). Monolayer adapted hPSCs exhibited SC-like morphology and maintained their expression of pluripotency markers (OCT4). More detailed characterization of expression of pluripotency markers in monolayer-adapted culture of hPSCs is shown in Supplemental Figure 1.