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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Stem Cell Res. 2016 Mar 29;16(3):640–650. doi: 10.1016/j.scr.2016.03.009

Figure 7. Optimized protocol for differentiation of hPSCs into HLCs within wells of a 384-well plate.

Figure 7

Non-colony-type cultures of hPSCs are differentiated into highly homogeneous definitive endoderm population. DE population homogeneity is assessed for co-expression of SOX17 and FOXA2. DE cells are then passed at a single-cell level on GF(-) Matrigel, before undergoing hepatic specification and maturation in a 384-well plate. At the different stage of hepatic specification and maturation, cells should be assessed for expression of hepatoblast marker AFP and hepatocyte marker ALB. One well of a 6-well plate of hPSCs usually produces over 1 full 384-well plate of highly homogeneous AFP(+) ALB(+) HLCs.