Fig. 2.

Anisotropic or isotropic alignment of hPSC-CMs cultured on the three substrates. (A) Schematic illustration of procedures used to directly differentiate hPSCs into CMs and to culture differentiated CMs on electrospun fibrous scaffolds or tissue culture polystyrenes (TCPs). After the cells were cultured on fibrous scaffolds or TCPs for 2 weeks, they were fixed directly and then subjected to immunocytochemical analysis of cell alignment. (B) Epi-/confocal-fluorescence micrographs of hPSC-CMs grown on various substrates for 14 days. Insets in (B): the corresponding phase-contrast images of hPSC-CMs cultured on various substrates. The white arrow indicated the alignment of fiber/cells. Scale bar: 20 µm.