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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Exp Eye Res. 2016 Apr 24;147:50–56. doi: 10.1016/j.exer.2016.04.012

Figure 4. Automated quantification method successfully captures microbead-induced optic neuropathy.

Figure 4

(A) Number of RGCs/mm2 counted using the CellProfiler automated quantification method in retinas isolated from non-injected (1616 ± 333 RGC/mm2, n = 6) and microbead-injected (1144 ± 452 RGC/mm2, n = 6) eyes. Values are presented as mean ± s.d. *P<0.05 vs. uninjected contralateral eye. (B) Number of RGCs/mm2 counted manually in retinas isolated from non-injected (3019 ± 643 RGC/mm2, n = 6) and microbead-injected (2404 ± 438 RGC/mm2, n = 6) eyes. (C) Results are correlated between manual and automated quantification methods of images (n = 162) from 12 murine retinas.