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. 2016 May 30;2016:6578578. doi: 10.1155/2016/6578578

Figure 4.

Figure 4

Comparison of (a) ADMA concentrations (nmol/g protein) determined by LC-MS/MS assays, (b) DDAH enzyme activity (nmol d7 ADMA/mg tissue/min) determined by LC-MS/MS assays, (c) mRNA expression of DDAH1 determined by qRT-PCR, (d) mRNA expression of DDAH2 determined by qRT-PCR, (e) DDAH1 protein expression levels determined by western blot, and (f) DDAH2 protein expression levels determined by western blot. Respective representative bands on western blots of lung tissue from rats exposed to normoxia (NX), chronic intermittent hypoxia (CIH2x2), or chronic hypoxia (CH) are presented. Values are expressed as means and SE. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; n = 8 in each group.