Figure 4.

MHCI activation and CD8⁺ T cell infiltration in the neuromuscular system of SOD1^G93A mice at disease onset. (A,B) Confocal micrographs of the sciatic nerve of C57SOD1^G93A mice at disease onset showing remarked upregulation of MHCI (green) and colocalization with Smi31 (axonal marker; red) in pathological conditions. (C,D^I) Confocal micrographs from tibialis anterior muscle of (C) Ntg and (D) C57-G93A mice showing accumulation of MHCI at the NMJ under pathological conditions (BTX; α-bungarotoxin; postsynaptic marker). (D^I) 3D rendering of (B) from the same angulation (Volocity – Perkin Elmer). (E–G) Confocal micrographs showing the presence cytototoxic T cells (CD8, green) in the tibialis anterior muscle of C57-G93A mice in proximity to NMJs, indicated by yellow arrows.