FIG. 1.

Role of macrophage CMs on the differentiation of 3T3-L1 preadipocyte. (A) Schematic of experimental design. Arrows point to days during adipocyte differentiation when CMs were first introduced. When CM is introduced on day 0, the cells were differentiated and kept until day 8, with change in media with CM every 2 days. When CM is introduced on day 3, the cells were grown in media with CM until day 8 with media changed every 2 days. When CM is introduced on day 12, the cells were grown in media with CM for 4 days (until day 16) with media change every 2 days. (B) mRNA levels of PPARγ and FABP4 in adipocytes from preadipocyte on day 0 to mature adipocytes on day 9. Mean ± SEM. n = 3 (C) mRNA expression of PPARγ and FABP4 in the adipocytes treated with 10% CMC or CMS on day 0 until day 8. Mean ± SEM, n = 3. Bars with different letters are significantly different (P < .05). CM, conditioned medium; CMC, conditioned medium control; CMS, conditioned medium spirulina; FABP4, fatty acid-binding protein 4; PPARγ, peroxisome proliferator-activated receptor γ.