FIG. 3.

Effects of macrophage CMs on lipid droplets in mature lipid-engorged adipocytes. 3T3-L1 adipocytes were treated with 10% CMC or CMS on day 12 for 4 days. (A) Micropictographs of 3T3-L1 adipocytes. From top to bottom, representative images of unstained cells (top panel), cells stained with Oil Red O (middle panel), and entire wells stained with Oil Red O (bottom). (B) mRNA expression of lipogenic and lipolytic genes. Mean ± SEM. n = 6. Bars with different letters for each gene are significantly different (P < .05). No letters in a gene indicates that there is no significant difference between groups. Color images available online at www.liebertpub.com/jmf