FIG. 4.

Effect of macrophage CM on NF-κB and IL-6 expression. 3T3-L1 adipocytes were incubated with 10% CMC or CMS on day 3 until day 5 or 7. (A) A representative blot of Western blot analysis of p65 in cytoplasmic and nuclear fractions. GAPDH and TBP were used as a control for cytoplasmic and nuclear fraction, respectively. Densitometry quantification of p65/TBP ratio relative to day 0 is shown. (B) IL-6 mRNA expression. Mean ± SEM. n = 6. Bars with different letters are significantly different (P < .05). GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; NF-κB, nuclear factor κB; TBP, TATA-binding protein.