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. 2016 Jun 13;6:27816. doi: 10.1038/srep27816

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Whole-mount in situ hybridization of zebrafish embryos at stages 15 hpf (a,b,h and i), 24 hpf (c–e,j–l) and 48 hpf (f,g,m,n). Anterior is to the left in all the whole-mount images, and dorsal is to the top in all transverse sections. (a,b). Lateral and dorsal view of the expression of kank1a at 15 hpf. (c,f). Lateral view of kank1a expression at 24 hpf and 48 hpf, respectively. (h,i). Lateral and dorsal view of the expression of kank1b at 15 hpf. (j,m). Lateral view of kank1b expression at 24 hpf and 48 hpf, respectively. The dashed lines indicate the positions of sections. The letters below the dashed lines correspond to the panels. b, brain; cf, caudal fin bud; di, diencephalon; e, eye; hb, hindbrain; hv, head blood vessels; kv, Kupffer’s vesicle; lpm, lateral plate mesoderm; mb, midbrain; n, notochord; nt, neural tube; op, optic vesicle; ov, otic vesicle; pa, pharyngeal arch; pf, pectoral fin.