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. 2016 Jun 13;6:27857. doi: 10.1038/srep27857

Figure 2. Generation of tissue-specific Creb3l3 knockout mice.

Figure 2

(a) Method used to generate tissue-specific CREB3L3 KO mice. (b) Genotyping of Creb3l3+/+ (+/+), Creb3k3 f lox/+ (f/+), and Creb3l3flox/flox (f/f) to confirm insertion of loxP sites into both Creb3l3 introns 3 and 11. (c) qPCR to measure hepatic and intestinal Creb3l3 expression in floxed, CREB3L3 LKO, and IKO mice in fasted condition (n = 5–6 per group). (d) Immunoblot analysis for CREB3L3 protein in hepatic and intestinal tissues from floxed, LKO, and IKO mice in fasted condition.