Figure 1. Subcellular localisation of TRIP6 and nTRIP6 in myoblasts.
(a) C2C12 cells were subjected to immunofluorescence analysis using an antibody recognising both TRIP6 and nTRIP6. Nuclei were counterstained using DRAQ5. (b) C2C12 cells were transfected with an empty vector (V) or an expression vector for either HA-tagged TRIP6 or nTRIP6, subjected to immunofluorescence analysis using an anti-HA antibody and counterstained using DRAQ5. (a,b) Representative confocal micrographs are shown, scale bars: 10 μm.