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. 2016 Jun 13;6:27746. doi: 10.1038/srep27746

Figure 5. nTRIP6 interacts with HDAC5.

Figure 5

(a) C2C12 cells were co-transfected with expression vectors for either HDAC5 or HDAC4 fused to the N-terminal half of Venus (VN) and for either nTRIP6 (wt), nTRIP6 LIM domains only (LIM) or nTRIP6 lacking the LIM domains (pre-LIM) fused to the C-terminal half of Venus (VC), together with an expression vector for mCherry as a transfection control. Venus complementation (Compl.) was imaged by confocal microscopy and representative cells are shown (top, scale bar: 20 μm). The number of cells showing Venus complementation is presented as percentage of transfected, mCherry positive cells (bottom, mean ± SD of three independent experiments; *P < 0.05). (b) C2C12 cells were co-transfected with a GAL4-responsive reporter gene together with an expression vector for β-galactosidase, with either an expression vector for nTRIP6 fused to the GAL4 DNA binding domain (GAL-nTRIP6; +) or an empty vector (−), and with either an expression vector for HDAC5 or an empty vector (V). Normalised luciferase activities are plotted relative to the empty vector control (mean ± SD of three independent experiments; *P < 0.05).