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. 2016 Jun 13;6:27746. doi: 10.1038/srep27746

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Copyright © 2016, Macmillan Publishers Limited

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(a) C2C12 cells were transfected with either an siRNA targeting Trip6 mRNA or a control siRNA (Co), and 24h later with expression vectors for HDAC5 fused to the N-terminal half of Venus (VN) and MEF2C fused to the C-terminal half of Venus (VC), together with an expression vector for mCherry as a transfection control. (b) C2C12 cells were co-transfected with expression vectors for HDAC5-VN and MEF2C-VC together with either the mCherry-NLS fusion of the ID2 peptide or its scrambled version (ID2c). (a,b) Venus complementation (Compl.) was imaged by confocal microscopy and representative cells are shown (top, scale bar: 20 μm). The number of cells showing Venus complementation is presented as percentage of transfected, mCherry positive cells (bottom, mean ± SD of three independent experiments; *P < 0.05).