Figure 7. nTRIP6 mediates the recruitment of HDAC5 to MEF2C target promoters.

(a) Chromatin immunoprecipitation (ChIP) was performed in C2C12 cells using the indicated antibodies (Ab). (b) Chromatin immunoprecipitated with the anti-TRIP6 antibody was eluted and subjected to a re-ChIP using either the anti-HDAC5 or the isotype control Ab. (a,b) PCR was performed with primers flanking the MEF2 binding regions of the indicted genes. Representative gels are shown. (c) C2C12 cells were transfected with either an siRNA targeting Trip6 mRNA or a control siRNA (Co) and ChIP was performed using either an anti-HDAC5 or an isotype control Ab. Enrichments of the MEF2 binding regions of the indicted genes were determined by real-time PCR and are plotted as percent input. A representative experiment is shown; repetitions are presented in Supplementary Fig. S5.