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. 2016 Jun 13;6:27819. doi: 10.1038/srep27819

Figure 6. Glipizide inhibits angiogenesis through down-regulation of ANGPT1 expression.

Figure 6

(A) ANGPT1 expression is down-regulated in HUVECs treated with glipizide by qRT-PCR array analysis. (B,C) To further determine the qRT-PCR array results, qRT-PCR (B) and Western blotting (C) were performed. (D,E) Immunofluorescent staining shows that the levels of ANGPT1 expression are high in the blood vessels of tumor tissues of TRAMP mice treated with DMSO. However, ANGPT1 expression is significantly decreased in the blood vessels of tumor tissues of TRAMP mice treated with glipizide. (F) ANGPT1 silencing and glipizide significantly inhibit HUVEC tube formation. In addition, glipizide does not affect the ability of HUVECs to form tubular structures after ANGPT1 is silenced. Scale bars, 20 μm in E and 50 μm in F. *p < 0.05, **p < 0.01, ***p < 0.001.