Figure 14. Expression of LC3-II and P62 in HepG2 cells with As₂O₃.

(A) Before incubation with 1 μM As₂O₃ for 24 h, the cells were pretreated with 100 μM RGS and 20 μM Tau respectively for 6 h. The protein fraction was analyzed by Western blot. β-actin was taken as internal control. (B) Densitometry analyses of LC3-II expressed in As₂O₃-treated HepG2 cells (**P < 0.01 vs. control; ^##P < 0.01). (C) The expression of P62 in HepG2 cells with As₂O₃. Before incubation with 1 μM As₂O₃ for 24 h, the cells were pretreated with 100 μM RGS and 20 μM Tau respectively for 6 h. The P62 protein fraction was analyzed by Western blot. β-actin was taken as internal control. (D) Densitometry analyses of P62 expressed in As₂O₃-treated HepG2 cells (*P < 0.05, **P < 0.01 vs. control; ^##P < 0.01).